The former Barcelona boss took charge of La Roja last year after their World Cup exit to Russia, but has missed their past three internationals having been on leave.
Former UEFA head Michel Platini is released after police questioning
Enrique’s assistant, Robert Moreno, will step up to become head coach, it was also confirmed.
Spain announced Enrique’s absence from their Euro 2020 qualifier against Malta back in March, citing ‘an urgent family matter’.
He also missed recent clashes with the Faroe Islands and Sweden, and RFEF president Luis Rubiales confirmed the managerial changes on Wednesday afternoon.
Speaking at a press conference, Rubiales said: “Luis Enrique announces that he will not continue as coach, and you have respected this situation a lot and continue to respect him in the same way.”
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